DYNC1LI2 encodes dynein cytoplasmic 1 light intermediate chain 2, a non-catalytic accessory subunit of the cytoplasmic dynein complex that mediates cargo-specific binding and transport functions 1. The protein exhibits distinct functional specificity compared to its paralog DYNC1LI1, with DYNC1LI2 primarily localizing to recycling endosomes and regulating their distribution, while also being essential for proper cytokinesis 2. During cell division, DYNC1LI2 specifically localizes to spindle poles from prophase through telophase, distinguishing it from DYNC1LI1 which localizes to the mitotic spindle and midbody 3. The protein undergoes post-translational regulation through arginine methylation at residue 397 by PRMT1, providing a mechanism for functional diversification of dynein complexes 4. DYNC1LI2 plays crucial roles in cellular homeostasis by regulating endolysosomal dynamics, chaperone-mediated autophagy through LAMP2A localization, and mitochondrial function 5. It interacts with membrane-associated proteins like Rab11-FIP3 to coordinate endosomal trafficking 6. Clinical relevance includes mapping to the SPG35 hereditary spastic paraplegia locus, though disease-causing mutations remain unidentified 7, and involvement in colorectal cancer progression as a potential hub protein 8.