GAS2L1 (growth arrest specific 2 like 1) is a cytolinker protein that functions as a crucial regulator of cytoskeletal dynamics and centrosome biology. The protein contains calponin-homology (CH) and GAS2-related (GAR) domains that bind F-actin and microtubules respectively, enabling cross-linking between these cytoskeletal networks 1. GAS2L1 exhibits autoinhibition through intramolecular interactions between its CH and GAR domains, which is relieved upon simultaneous binding to both actin filaments and microtubules 2. In centrosome biology, GAS2L1 localizes to the proximal end of mature centrioles and is essential for centrosome disjunction during late G2 phase 3. This function is regulated by Nek2-mediated phosphorylation at Ser352, which disrupts the autoinhibition and enables proper spindle assembly and chromosome 22 1. In neurons, GAS2L1 promotes axon branching while restricting axon elongation by stabilizing the actin cytoskeleton in a microtubule-dependent manner 2. The protein shows clinical relevance in multiple contexts: it is upregulated in hepatocellular carcinoma where it promotes cell proliferation and serves as a prognostic marker 4, and its expression is elevated in diabetic nephropathy through AGE-RAGE signaling 5. Additionally, mTORC1-mediated phosphorylation of GAS2L1 participates in centriole disengagement during ependymal cell differentiation 6.