GOT2 (glutamic-oxaloacetic transaminase 2) is a mitochondrial enzyme that catalyzes transamination reactions central to cellular metabolism and signaling. Its primary function involves the malate-aspartate shuttle, maintaining intracellular NAD(H) redox balance and facilitating metabolite exchange between mitochondria and cytosol 1. GOT2 catalyzes conversion of aspartate and glutamate, supporting amino acid metabolism and nucleotide synthesis 2. Beyond canonical metabolic functions, GOT2 exhibits moonlighting properties. It directly binds fatty acids to regulate the nuclear receptor PPARδ, influencing transcriptional control of immune evasion in pancreatic cancer 3. Cytoplasmic GOT2 can be enhanced by lncRNA-ACOD1 binding, which promotes viral replication through metabolic reprogramming 4. In hepatocellular carcinoma, GOT2 downregulation promotes glutamine metabolic reprogramming, enhancing glutaminolysis and antioxidant synthesis via PI3K/AKT/mTOR pathway activation, conferring sensitivity to glutaminase inhibitors 2. GOT2 dysfunction associates with hypoalphalipoproteinemia (low HDL-cholesterol), with damaging variants identified in affected individuals 5. Additionally, GOT2 serves as a biomarker for systemic lupus erythematosus disease exacerbation assessment 6. GOT2 mutations cause developmental and epileptic encephalopathy 82, underscoring its clinical importance in neurological disease.