KIF24 is a microtubule-dependent motor protein that functions as a negative regulator of ciliogenesis in cycling cells. It belongs to the kinesin-13 subfamily and specifically localizes to mother centrioles, where it interacts with centrosomal proteins CP110 and Cep97 1. KIF24 mediates the recruitment of CCP110 to mother centrioles and directly depolymerizes centriolar microtubules, suppressing aberrant cilia formation 1. During cell cycle progression, the S/G2 kinase NEK2 phosphorylates and activates KIF24, promoting cilium disassembly during G2/M phase while maintaining the disassembled state through dynamic equilibrium shifts toward microtubule depolymerization 2. KIF24 also recruits MPHOSPH9, another negative regulator of cilia formation, to the distal end of mother centrioles 1. Beyond ciliogenesis regulation, KIF24 plays a centrosome clustering role independent of primary cilia in cancer cells with supernumerary centrosomes 3. Disease associations include Parkinson's disease, where KIF24 was identified as a novel candidate gene with disrupting variants showing significant excess in PD patients 4, and potential involvement in frontotemporal lobar degeneration 5. KIF24 overexpression in breast cancer cells promotes proliferation by suppressing ciliation 2.