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10 sources retrieved · Most recent: April 2026 · Index updated 15 days ago
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NCEH1
neutral cholesterol ester hydrolase 1
Chromosome 3 · 3q26.31
NCBI Gene: 57552Ensembl: ENSG00000144959.11HGNC: HGNC:29260UniProt: A0A0A0MTJ9
52PubMed Papers
20Diseases
0Drugs
0Pathogenic Variants
DATA QUALITY
✓ Experimental GO Evidence✓ Swiss-Prot Reviewed
membraneether lipid metabolic processserine hydrolase activitysterol ester esterase activityprostate carcinomasmoking initiationbilirubin metabolism diseasepreeclampsia
✦AI Summary

NCEH1 (neutral cholesterol ester hydrolase 1) is a serine hydrolase that catalyzes the hydrolysis of cholesterol esters to free cholesterol and fatty acids in macrophages 1. This enzyme plays a critical role in cholesterol homeostasis by generating free cholesterol that is preferentially effluxed from cells via ABC transporters, thereby protecting against foam cell formation 2. NCEH1 is also involved in platelet-activating factor synthesis by hydrolyzing 2-acetyl monoalkylglycerol ether and functions in organophosphate detoxification [UniProt annotation]. In atherosclerosis, NCEH1 expression is dysregulated; elevated NCEH1 promotes cholesterol accumulation in tumor microenvironments and contributes to disease progression 3. NCEH1 is upregulated in pancreatic cancer and associated with poor overall survival 4, while activity-based PET imaging reveals NCEH1 localization at the invasive front of triple-negative breast cancers, suggesting roles in tumor aggressiveness and metastasis 5. Cadmium exposure downregulates NCEH1 expression via miR-30d-5p, disrupting cholesterol homeostasis and promoting atherosclerosis 6. Thus, NCEH1 functions as a dual-role enzyme: protective in atherosclerosis prevention but pathogenic when dysregulated in cancer progression.

Sources cited
1
AURKB regulates NCEH1 expression and cholesterol levels in cholangiocarcinoma; high AURKB/NCEH1 predicts poor outcomes in chemoimmunotherapy
PMID: 40744724
2
NCEH1 is part of a 5-gene prognostic risk score for head and neck squamous cell carcinoma overall survival
PMID: 36076232
3
NCEH1 is upregulated in pancreatic cancer and significantly associated with overall survival of pancreatic cancer patients
PMID: 34078402
4
NCEH1 hydrolyzes cholesterol esters in macrophages to liberate free cholesterol for efflux via ABC transporters, preventing foam cell formation
PMID: 26493158
5
Activity-based PET imaging shows NCEH1 localizes at the leading edge of triple-negative breast cancer tumors, suggesting roles in tumor aggressiveness and metastasis
PMID: 32415874
6
Cadmium exposure downregulates NCEH1 via miR-30d-5p, disrupting cholesterol homeostasis and promoting atherosclerosis
PMID: 41297938
7
NCEH1 mediates hydrolysis of cholesterol esters in macrophages to generate free cholesterol for efflux, protecting against foam cell formation and atherosclerosis
PMID: 21467808
Disease Associationsⓘ20
prostate carcinomaOpen Targets
0.38Weak
smoking initiationOpen Targets
0.32Weak
bilirubin metabolism diseaseOpen Targets
0.30Weak
preeclampsiaOpen Targets
0.30Weak
neurodegenerative diseaseOpen Targets
0.29Weak
connective tissue diseaseOpen Targets
0.29Weak
atrial heart septal defectOpen Targets
0.11Weak
response to antihypertensive drugOpen Targets
0.11Weak
gastric cancerOpen Targets
0.07Suggestive
vascular diseaseOpen Targets
0.07Suggestive
renal carcinomaOpen Targets
0.04Suggestive
colorectal carcinomaOpen Targets
0.04Suggestive
medulloblastomaOpen Targets
0.04Suggestive
placental retentionOpen Targets
0.04Suggestive
glaucomaOpen Targets
0.03Suggestive
HydrocephalusOpen Targets
0.03Suggestive
ovarian dysfunctionOpen Targets
0.03Suggestive
pachyonychia congenitaOpen Targets
0.03Suggestive
ovarian cancerOpen Targets
0.03Suggestive
breast cancerOpen Targets
0.03Suggestive
Pathogenic Variants
No pathogenic variants reported on ClinVar for this gene.
View on ClinVar ↗
Related Genes
CES1Protein interaction96%MGLLProtein interaction96%TMEM86BShared pathway33%LIPAShared pathway33%CES3Shared pathway33%AGMOShared pathway25%
Tissue Expression6 tissues
Brain
100%
Heart
86%
Bone Marrow
58%
Lung
28%
Liver
7%
Ovary
6%
Gene Interaction Network
Click a node to explore
NCEH1CES1MGLLTMEM86BLIPACES3AGMO
PROTEIN STRUCTURE
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AlphaFoldAI-predicted · UniProt Q6PIU2
View on AlphaFold ↗
Constraintⓘ
LOEUFⓘ
0.53Moderately Constrained
pLIⓘ
0.96Intolerant
Observed/Expected LoF0.28 [0.16–0.53]
RankingsWhere NCEH1 stands among ~20K protein-coding genes
  • #8,587of 20,598
    Most Researched52
  • #3,307of 17,882
    Most Constrained (LOEUF)0.53 · top quartile
Genes detectedNCEH1
Sources retrieved10 papers
Response time—
📄 Sources
10▼
1
Targeting Aurora kinase B regulates cholesterol metabolism and enhances chemoimmunotherapy in cholangiocarcinoma.
PMID: 40744724
Gut · 2026
1.00
2
A transcriptomic map of EGFR-induced epithelial-to-mesenchymal transition identifies prognostic and therapeutic targets for head and neck cancer.
PMID: 36076232
Mol Cancer · 2022
0.90
3
Identification of prognostic lipid droplet-associated genes in pancreatic cancer patients via bioinformatics analysis.
PMID: 34078402
Lipids Health Dis · 2021
0.80
4
Macrophage-mediated cholesterol handling in atherosclerosis.
PMID: 26493158
J Cell Mol Med · 2016
0.70
5
Lipid droplet-associated hydrolase mobilizes stores of liver X receptor sterol ligands and protects against atherosclerosis.
PMID: 39095402
Nat Commun · 2024
0.60