NDUFB8 (NADH:ubiquinone oxidoreductase subunit B8) is an accessory subunit of mitochondrial respiratory complex I that plays crucial roles in oxidative phosphorylation and cellular energy metabolism 1. As a component of complex I, NDUFB8 participates in the electron transport chain, facilitating NADH oxidation and contributing to ATP synthesis through oxidative phosphorylation 23. The protein localizes to the mitochondrial inner membrane and is essential for proper complex I assembly and function 4. NDUFB8 expression serves as a biomarker in various pathological contexts, including cancer prognosis and treatment response. In pancreatic ductal adenocarcinoma, NDUFB8 levels predict sensitivity to adjuvant chemotherapy 5. In renal cell carcinoma, high NDUFB8 expression correlates with immune checkpoint inhibitor resistance by promoting a hypoxic tumor microenvironment that impairs T-cell function 3. Similarly, in African American bladder cancer patients, elevated NDUFB8 drives glutamine-mediated metabolic reprogramming and increased disease progression 2. The gene is associated with mitochondrial complex I deficiency when mutated 1, and its expression is regulated during cellular stress responses, including autophagy and mitochondrial quality control mechanisms 67. NDUFB8 also shows cardioprotective potential, with its upregulation contributing to protection against myocardial ischemia-reperfusion injury 4.