NXF2 (nuclear RNA export factor 2) is a member of the evolutionarily conserved NXF family involved in mRNA transport from nucleus to cytoplasm 1. Unlike the ubiquitously expressed NXF1, NXF2 shows tissue-specific expression patterns, being highly expressed in testis and brain regions 12. Mechanistically, NXF2 functions through interactions with NXT proteins to form heterodimeric complexes essential for mRNA export, with NXT2 serving as a key cofactor that binds NXF2 via its NTF2-like domain 2. The protein exhibits nuclear localization and can enhance nuclear export of mRNA substrates 1. Beyond nuclear export, NXF2 participates in cytoplasmic mRNA dynamics through interactions with motor proteins like KIF17, suggesting roles in mRNA transport within neurons 3. Clinically, NXF2 has significance as a cancer-testis antigen that becomes aberrantly activated in acute leukemia through DNA hypomethylation, potentially contributing to genome instability and serving as an immunotherapy target 45. Loss-of-function variants in NXF2's cofactor NXT2 cause male infertility by disrupting germ cell development, highlighting the critical role of this export machinery in spermatogenesis 2.