PRAG1 (PEAK1-related kinase-activating pseudokinase 1) is a catalytically inactive scaffold protein that regulates cell morphology, migration, and stress responses through protein-protein interactions rather than kinase activity. PRAG1 forms dynamic phase-separated condensates mediated by its αN and αJ helices, which drive cell contraction under stress conditions 1. The protein functions as a nodal signaling hub: it activates Src family kinase signaling by sequestering CSK (a negative regulator) away from its targets, and promotes epithelial-mesenchymal transition (EMT) by binding FBXO11 and stabilizing SNAIL1, a master EMT transcription factor 2. In colorectal cancer, high PRAG1 expression correlates with poor prognosis and promotes cell invasion through ABL kinase activation and filopodia formation 3. Disease relevance spans multiple conditions: PRAG1 genetic variants associate with IgA nephropathy (rs143409664) 4, cardiac allograft vasculopathy (rs11785239) 5, asthma bronchodilator response (rs4840337) 6, and major depressive disorder treatment response 7. Notably, aberrant PRAG1 condensation occurs in Parkinson's disease dopaminergic neurons, suggesting links between PRAG1 dysfunction and neurodegenerative pathology 1. These findings establish PRAG1 as a multifunctional adapter protein controlling diverse cellular processes with significant clinical implications.