PRR5L (proline rich 5 like) is a regulatory protein that associates with the mTORC2 complex and controls multiple cellular processes. PRR5L binds specifically to mTORC2 via Rictor and/or SIN1, where it regulates the activity of the mTORC2 complex in a substrate-specific manner, preventing specific phosphorylation of protein kinase C (PKC) and thereby controlling cell migration 1. Unlike other mTORC2 components, PRR5L is not required for mTORC2 integrity or kinase activity but dissociates from mTORC2 upon TSC1/2 knockdown 1. The protein plays a role in apoptosis regulation, with PRR5L knockdown reducing apoptosis even in mTORC2-deficient cells, suggesting mTORC2-independent functions 1. PRR5L demonstrates clinical relevance through epigenetic regulation, as DNA methylation of PRR5L has been associated with body mass index in multiple populations 2 and shows differential methylation patterns in response to soy formula exposure in infants 3. The gene appears downregulated in osteoarthritis 4 and shows expression changes associated with breast cancer progression 5, suggesting broader roles in disease pathogenesis.