RPL35A encodes a ribosomal protein that is a structural component of the large 60S ribosomal subunit, playing an essential role in protein synthesis 1. The protein functions as a structural constituent of the cytosolic ribosome and is involved in ribosomal large subunit biogenesis and cytoplasmic translation 1. Beyond its canonical ribosomal function, RPL35A exhibits extraribosomal roles in cancer progression. In cholangiocarcinoma, RPL35A promotes tumor progression by mediating HSPA8 ubiquitination, leading to increased cell proliferation and migration 2. Similarly, in ovarian cancer, RPL35A drives cancer progression by promoting the binding of transcription factor YY1 to the CTCF promoter 3. In hepatocellular carcinoma, RPL35A overexpression correlates with poor prognosis and promotes tumor growth through NCAPG2 activation 4. The protein also shows tissue-specific upregulation in Alzheimer's disease brain capillaries, where ribosomal protein complexes are significantly elevated 1. RPL35A expression is involved in muscle hypertrophy memory through epigenetic mechanisms 5 and may contribute to orofacial clefting development 6. The gene is located on chromosome 3-qter 7.