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10 sources retrieved · Most recent: April 2026 · Index updated 15 days ago
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TRIM35
tripartite motif containing 35
Chromosome 8 · 8p21.2
NCBI Gene: 23087Ensembl: ENSG00000104228.13HGNC: HGNC:16285UniProt: E5RGB3
35PubMed Papers
20Diseases
0Drugs
0Pathogenic Variants
DATA QUALITY
✓ Experimental GO Evidence✓ Swiss-Prot Reviewed
positive regulation of apoptotic processsuppression of viral release by hostinnate immune responsenegative regulation of mitotic cell cyclerestless legs syndromeTietze syndromeRaynaud diseasehepatocellular carcinoma
✦AI Summary

TRIM35 is an E3 ubiquitin ligase with pleiotropic functions in innate immunity, metabolism, and disease pathogenesis. In immune regulation, TRIM35 mediates K63-linked polyubiquitination of TRAF3 to promote type I interferon production via RIG-I signaling 1 and catalyzes K48-linked ubiquitination of viral proteins like influenza PB2 1. It acts as a negative regulator of TLR7/TLR9 signaling by promoting IRF7 degradation 2. TRIM35 also negatively regulates cGAS-STING signaling by deubiquitinating STING, attenuating antiviral responses 3. Additionally, TRIM35 inhibits human adenovirus replication through E1A degradation 4. In metabolic regulation, TRIM35 reduces PKM2-dependent lactate production and glucose metabolism 5, suppressing the Warburg effect in breast cancer 6. It promotes amino acid transporter SLC7A5 ubiquitination in cardiac fibroblasts, enhancing mTORC1 signaling and contributing to cardiac remodeling 7. TRIM35 also monoubiquitinates histone H2B-K120 in cardiomyocytes, promoting P53 transcriptional activity and heart failure pathogenesis 8. Clinically, low TRIM35 expression correlates with poor prognosis in breast cancer and hepatocellular carcinoma 69, while TRIM35 expression predicts favorable immunotherapy outcomes in NSCLC 10. Endothelial TRIM35 suppresses vascular calcification by inhibiting MMP10 secretion 11.

Sources cited
1
In immune regulation, TRIM35 mediates K63-linked polyubiquitination of TRAF3 to promote type I interferon production via RIG-I signaling and catalyzes K48-linked ubiquitination of viral proteins like influenza PB2 .
PMID: 32562145
2
It acts as a negative regulator of TLR7/TLR9 signaling by promoting IRF7 degradation .
PMID: 25907537
3
TRIM35 also negatively regulates cGAS-STING signaling by deubiquitinating STING, attenuating antiviral responses .
PMID: 39088122
4
Additionally, TRIM35 inhibits human adenovirus replication through E1A degradation .
PMID: 37578239
5
In metabolic regulation, TRIM35 reduces PKM2-dependent lactate production and glucose metabolism , suppressing the Warburg effect in breast cancer .
PMID: 25263439
6
In metabolic regulation, TRIM35 reduces PKM2-dependent lactate production and glucose metabolism , suppressing the Warburg effect in breast cancer .
PMID: 36196894
7
It promotes amino acid transporter SLC7A5 ubiquitination in cardiac fibroblasts, enhancing mTORC1 signaling and contributing to cardiac remodeling .
PMID: 39304904
8
TRIM35 also monoubiquitinates histone H2B-K120 in cardiomyocytes, promoting P53 transcriptional activity and heart failure pathogenesis .
PMID: 38860363
9
Clinically, low TRIM35 expression correlates with poor prognosis in breast cancer and hepatocellular carcinoma , , while TRIM35 expression predicts favorable immunotherapy outcomes in NSCLC .
PMID: 37979167
10
Endothelial TRIM35 suppresses vascular calcification by inhibiting MMP10 secretion .
PMID: 39865905
Disease Associationsⓘ20
restless legs syndromeOpen Targets
0.33Weak
Tietze syndromeOpen Targets
0.32Weak
Raynaud diseaseOpen Targets
0.30Weak
hepatocellular carcinomaOpen Targets
0.10Suggestive
breast cancerOpen Targets
0.08Suggestive
diffuse large B-cell lymphomaOpen Targets
0.08Suggestive
lung cancerOpen Targets
0.07Suggestive
Alzheimer diseaseOpen Targets
0.06Suggestive
Takayasu arteritisOpen Targets
0.05Suggestive
contact dermatitisOpen Targets
0.04Suggestive
liver cancerOpen Targets
0.04Suggestive
neoplasmOpen Targets
0.04Suggestive
infectionOpen Targets
0.04Suggestive
ovarian dysfunctionOpen Targets
0.04Suggestive
autosomal recessive nonsyndromic hearing loss 9Open Targets
0.04Suggestive
deafnessOpen Targets
0.04Suggestive
dementiaOpen Targets
0.03Suggestive
thyroid cancerOpen Targets
0.03Suggestive
Erythema nodosumOpen Targets
0.03Suggestive
autosomal dominant nonsyndromic hearing lossOpen Targets
0.03Suggestive
Pathogenic Variants
No pathogenic variants reported on ClinVar for this gene.
View on ClinVar ↗
Related Genes
TRIM15Shared pathway29%DEFB131BShared pathway25%DEFB131AShared pathway25%DEFB134Shared pathway25%BLIDShared pathway25%TRIM74Shared pathway25%
Tissue Expression6 tissues
Liver
100%
Bone Marrow
79%
Brain
74%
Heart
64%
Lung
63%
Ovary
54%
Gene Interaction Network
Click a node to explore
TRIM35TRIM15DEFB131BDEFB131ADEFB134BLIDTRIM74
PROTEIN STRUCTURE
Preparing viewer…
AlphaFoldAI-predicted · UniProt Q9UPQ4
View on AlphaFold ↗
Constraintⓘ
LOEUFⓘ
0.97LoF Tolerant
pLIⓘ
0.00Tolerant
Observed/Expected LoF0.74 [0.57–0.97]
RankingsWhere TRIM35 stands among ~20K protein-coding genes
  • #11,087of 20,598
    Most Researched35
  • #9,267of 17,882
    Most Constrained (LOEUF)0.97
Genes detectedTRIM35
Sources retrieved10 papers
Response time—
📄 Sources
10▼
1
Endothelial TRIM35-Regulated MMP10 Release Exacerbates Calcification of Vascular Grafts.
PMID: 39865905
Adv Sci (Weinh) · 2025
1.00
2
TRIM35 triggers cardiac remodeling by regulating SLC7A5-mediated amino acid transport and mTORC1 activation in fibroblasts.
PMID: 39304904
Cell Commun Signal · 2024
0.90
3
E3 ligase Trim35 inhibits LSD1 demethylase activity through K63-linked ubiquitination and enhances anti-tumor immunity in NSCLC.
PMID: 37979167
Cell Rep · 2023
0.80
4
TRIM35 ubiquitination regulates the expression of PKM2 tetramer and dimer and affects the malignant behaviour of breast cancer by regulating the Warburg effect.
PMID: 36196894
Int J Oncol · 2022
0.70
5
Inhibition of human adenovirus replication by TRIM35-mediated degradation of E1A.
PMID: 37578239
J Virol · 2023
0.60