ZNF25 (zinc finger protein 25) is a KRAB-containing transcription factor located in the pericentromeric region of human chromosome 10 1 that functions as a DNA-binding transcriptional repressor of RNA polymerase II [GO annotations]. The protein exhibits nuclear localization and is expressed in osteoblastic and osteocytic cells in vivo 2. Mechanistically, ZNF25 acts as a transcriptional repressor during osteoblast differentiation. siRNA knockdown of ZNF25 significantly suppressed alkaline phosphatase activity in human mesenchymal stem cells 2, revealing its role in regulating genes involved in extracellular matrix organization and skeletal system development. ZNF25 appears to repress matrix metallopeptidase 1 and other genes associated with bone remodeling 2. Clinically, ZNF25 has emerged as a candidate gene in multiple disease contexts. Genome-wide association meta-analysis identified ZNF25 as a novel susceptibility locus for Hirschsprung disease (HSCR), a congenital disorder affecting enteric nervous system development, with functional studies demonstrating that ZNF25 knockdown impairs cell migration and zebrafish knockouts display abnormal ENS development 3. Additionally, ZNF25 has been identified as a putative target in gestational diabetes mellitus pathology through microRNA-mediated regulation 4 and as a key lncRNA target in Parkinson's disease 5, suggesting broader roles in metabolic and neurological processes.