CNOT8 is a catalytic subunit of the CCR4-NOT deadenylase complex with 3'-5' poly(A) exoribonuclease activity 1. As part of the CCR4-NOT core complex, CNOT8 mediates mRNA degradation through deadenylation, the removal of poly(A) tails from mRNAs 2. CNOT8 functions in multiple interconnected processes: it participates in miRNA-mediated gene silencing through direct recruitment by YTHDF2 to destabilize m(6)A-modified RNAs 3, and it exhibits partial functional redundancy with CNOT7 in regulating cell proliferation 1. CNOT8 interacts with anti-proliferative BTG family proteins to modulate their biological activities 4. Beyond mRNA decay, CNOT8 contributes to induced pluripotent stem cell generation through deadenylation-dependent regulation of reprogramming-associated mRNAs 5, and it plays a role in cellular responses to ionizing radiation-induced DNA damage 6. Clinically, CNOT8 expression has been identified as a prognostic factor in clear cell renal cell carcinoma immunotherapy outcomes 7. The complex functions of CNOT8 reflect its central role in post-transcriptional gene expression control across diverse cellular contexts.