DACT3 (dishevelled binding antagonist of beta catenin 3) functions as a negative regulator of canonical Wnt/β-catenin signaling through direct interaction with dishevelled (DVL) family proteins 12. The protein suppresses Wnt signaling by inhibiting DVL-mediated phosphorylation of GSK-3β and β-catenin, thereby preventing β-catenin nuclear translocation and downstream transcriptional activation 12. DACT3 demonstrates tumor suppressive properties across multiple cancer types, including acute myeloid leukemia, non-small cell lung cancer, and colorectal cancer, where it inhibits cell proliferation, migration, invasion, and promotes apoptosis 123. In colorectal cancer, DACT3 expression is epigenetically silenced by bivalent histone modifications rather than DNA methylation 3. The gene also plays roles in cellular processes beyond cancer, as DACT3 upregulation by the beneficial bacterium Faecalibacterium prausnitzii mediates anti-inflammatory effects in intestinal epithelial cells through butyrate signaling 4. Additionally, DACT3 contributes to vascular health by inhibiting vascular smooth muscle cell proliferation and migration 5. Clinically, reduced DACT3 expression correlates with poor prognosis and chemotherapy resistance in various cancers 21.