POP7 is a protein subunit of ribonuclease P and ribonuclease MRP complexes, essential ribonucleoprotein enzymes involved in RNA processing. As a component of ribonuclease P, POP7 participates in generating mature tRNA molecules by cleaving their 5' leader sequences 12. It also functions as part of the MRP ribonuclease complex, which processes pre-rRNA sequences critical for ribosomal biogenesis 3. Structurally, POP7 interacts with the P3 RNA domain, a conserved element unique to eukaryotic RNases P/MRP 45. Beyond its canonical roles in tRNA and rRNA processing, POP7 exhibits functions as an RNA-binding protein with broader regulatory capabilities. POP7 is frequently overexpressed in breast cancer tissues and cells, where it promotes malignancy through regulation of ILF3 mRNA stability, enhancing cancer cell proliferation, migration, invasion, and metastatic potential 6. Additionally, POP7 serves as a reliable internal control gene in molecular diagnostics for SARS-CoV-2 and tuberculosis detection, where its consistent expression makes it suitable for validating nucleic acid extraction and amplification procedures 789. These findings suggest POP7 has expanded biological roles beyond canonical RNA processing, implicating it in cancer progression and establishing its utility in clinical diagnostics.