RPL35 is a ribosomal protein that serves as a component of the large ribosomal subunit (60S), playing essential roles in protein synthesis and cellular regulation 123. Beyond its canonical ribosomal function, RPL35 exhibits diverse regulatory activities. It interacts with long non-coding RNA lncNB1 to enhance E2F1 protein synthesis, promoting neuroblastoma tumorigenesis through N-Myc protein stabilization 4. In hepatocellular carcinoma, RPL35 interacts with TENT5A to regulate ribosome biogenesis and negatively modulate the mTOR pathway, with reduced RPL35 stability associated with cancer progression 5. RPL35 also demonstrates antiviral properties by recruiting the E3 ligase AMFR to promote K48-linked polyubiquitination and degradation of viral proteins, as shown with foot-and-mouth disease virus VP2 6. In colorectal cancer, DDX10 promotes proliferation and metastasis through splicing-mediated regulation of RPL35 7. Additionally, RPL35 serves as a drug target for epidermolysis bullosa therapy, where small molecules can bind to modify its ribosomal function to enhance production of functional LAMB3 protein from mutant mRNA 8. RPL35 is also implicated in Alzheimer's disease pathology through ceRNA networks 9.