SPC24 is a structural component of the NDC80 kinetochore complex essential for mitotic chromosome 19. As a core kinetochore protein, SPC24 is required to establish and maintain kinetochore-microtubule attachments and organize stable microtubule binding sites in the outer kinetochore plate 1. The NDC80 complex containing SPC24 synergistically enhances microtubule binding affinity through interaction with the SKA1 complex and enables tracking of depolymerizing microtubules during anaphase 1. Beyond its canonical mitotic role, SPC24 participates in pre-rRNA transcription and ribosome biogenesis during interphase through interactions with nucleolar machinery, with SPC24 depletion reducing POLR1A levels and activating nucleolar stress pathways 2. Clinically, SPC24 overexpression associates with poor prognosis across multiple malignancies. In hepatocellular carcinoma, elevated SPC24 correlates with advanced tumor stage, increased invasion, and shortened survival; SPC24 silencing suppresses HCC cell growth and invasion 3. Similarly, SPC24 upregulation in laryngeal squamous cell carcinoma and renal carcinomas (KIRC/KIRP) predicts worse outcomes and correlates with immune infiltration and tumor mutational burden 456. In osteosarcoma, SPC24 promotes progression via EGFR/MAPK signaling activation 7. These findings establish SPC24 as a pan-cancer prognostic biomarker with potential therapeutic targeting value.